GST fusions and antibodies

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Sep 12 04:14:30 EST 1997


Mara Michelle Casar (mmc9 at acpub.duke.edu) wrote:
> I am trying to get some information about producting antibodies to GST 
> fusion proteins.  I have two proteins I want to send off for 
> immunizations, but I need advice first:
> 
> 1.  To cleave or not to cleave?  My fused piece is about 9 kD, and seems 
> pretty stable, but is it better to send the whole protein, which should 
> be more immunogenic?

However, you would then have to purify your serum over a GST-GSH column
since you will get a lot of anti-GST.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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