concentration of low molecular weight protein

Stephen C. Dahl stebby at welchlink.welch.jhu.edu
Fri Sep 19 17:53:45 EST 1997


Damn this server....somebody wrote:

: How do I concentrate a low molecular weight protein (less than 5kDa)
: from gel filtration fractions ie. dilute???, keeping in mind that
: filtration devices and dialysis tubing concentrate above this size.


One option which I've used on a limited basis (and have never taken the
time to quantify the results) is to swell a powdered gel filtration
medium--in your case Sephadex G10 or G15--in your pooled fractions.  The
beads take up the water and, in general, exclude your protein if you
choose your matrix wisely.  Good luck.

Steve Dahl



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