concentration of low molecular weight protein
stebby at WELCHLINK.WELCH.JHU.EDU
Thu Sep 25 18:07:30 EST 1997
On Sat, 20 Sep 1997, Hiranya Roychowdhury wrote:
> I wonder if extracting the protein solution with something like n-butanol
> will do the trick too. I have used it to concentrate DNA solutions. But
> then, some proteins may ppt in that solvent. If this one doesn't, then
> surely n-butanol extraction is worth a try. The other alternative is to use
> a volatile buffer (eg. bicarbonate) in protein elution and then to
> freeze-dry the sample.
Yeah--I forgot about the butanol method. I vaguely remember trying it
with protein a few years ago and not being too thrilled with the results,
but that could have been because I forgot to take into consideration the
concentration of salt, etc. that would also occur. I agree that it would
be worth a shot. The bicarb/lyophilization idea is right on if the
protein resolubilizes readily and retains any desired activity.
Thanks for the follow-up!
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