Peptide fixation

Betsy Lytle lytle at CHE.ROCHESTER.EDU
Fri Sep 26 07:49:42 EST 1997


I forgot to mention in my post yesterday that I am using Tricine gels.

>Hi all,
>
>I am analyzing a 3.5 kD protein (pI=9.3) by SDS-PAGE and am having problems
>visualizing it. This could be due either to poor staining or to diffusion
>of the protein out of the gel. Staining by Coomassie blue and silver stain
>are both poor, which makes me think diffusion is the problem. I have tried 
>fixing the gel with 12% TCA/3.5% sulfosalicylic acid without success. I may
>try using glutaraldehyde next, but would like any advice from others who
>have had this problem.
>
>Thanks,
>
>Betsy Lytle 



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