Anti-His tag antibodies
mmm_klehnert at mednov1.auckland.ac.nz
Sun Sep 28 20:16:49 EST 1997
In article , says...
>Has anyone out there had any success using anti-His tag
antibodies for western blots?
I'm just testing a sample from Boehringer Mannheim (anti-His6,
mouse IgG1). It looks perfect in my Western Blots, doesn't
interfere with my blocking solutions (BSA, Tween or NF milk
based) and comes also conjugated with HRPO. Did so far detect
C-terminally 6-His tagged proteins with 4 different flanking
sequences. Didn't test N-terminally tagged proteins, but the
BM's say it sees them as well. Don't know whether they are in
the catalogue yet, but check themon the web or ring your rep.
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