cell fractionation

Tilman Voss voss at bender.co.at
Tue Sep 30 19:51:33 EST 1997


hi out there
I am trying to fractionate lymphocytic/lymphatic cells into a nuclear, a
membrane/mitochondria and a cytoplasmic fraction. these cells are pretty
tough to crack and fractionate. I do observe quite som leaking of
mitochondrial markers (CytC oxidase)´when breaking them up with a
Douance or by frezze/thaw cycles or a french press.
Does anyone have a good protocol or suggestions?

Thanks Tilman



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