antibodies/DTT

Simon Easterbrook-Smith sbe at biochem.usyd.edu.au
Mon Sep 29 19:01:42 EST 1997


It depends which ones you want to break. At least in rabbit IgG the interH
chain -SS- bond can be cleaved with about 0.1-0.2 mM DTT at pH 8, while the
interHL bonds need about 1 mM DTT. In the absence of denaturants, the
intra-domain bonds  are relatively resistant.




In article <mbzrl-290997145303 at pmbfjd0.nottingham.ac.uk>,
mbzrl at mbn1.biochem.nottingham.ac.uk (Rob) wrote:

|| Hi
|| Can anyone suggest the concentration of DTT (or mercaptoethanol) and
|| conditions which would be needed to break the disulphides in an antibody.
|| Thanks
|| Rob

Simon
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