cross reactivty on Western immunoblotting

Dr E. Buxbaum EB15 at le.ac.uk
Mon Aug 3 17:01:34 EST 1998


david pryce wrote:
> Can anyone tell me if there are any "cheap" alternatives to using milk > powder as a blocking agent 

A former boss of mine swore by fish skin gelatine, a liquid of the
colour and consitency of used motor oil, but freely soluble in PBS. The
stuff is available from Sigma, but be carefull: their bottle cap is ill
designed and you won't be able to open it a second time. BSA or common
gelatine are also possible.



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