krasel at wpxx02.toxi.uni-wuerzburg.de
Tue Aug 4 09:06:50 EST 1998
Lars Komorowski <larskomo at physik.mu-luebeck.de> wrote:
>>If your protein is unstable, it will not survive overnight induction.
>>Can you detect your protein by western blotting?
> No, I can not. But I find a very huge band at 25kDa and it is
Have you looked for this band in cells which were transformed with
empty pET vector? This is usually the best control since induced
beta-galactosidase, T4 RNA polymerase etc. will show up there as
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
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