pEt-Expression-Mystery

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Tue Aug 4 09:06:50 EST 1998


Lars Komorowski <larskomo at physik.mu-luebeck.de> wrote:
>>If your protein is unstable, it will not survive overnight induction.
>>Can you detect your protein by western blotting?
> 
> No, I can not. But I find a very huge band at 25kDa and it is
> IPTG-dependant.

Have you looked for this band in cells which were transformed with
empty pET vector? This is usually the best control since induced
beta-galactosidase, T4 RNA polymerase etc. will show up there as
well.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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