How to get rid of DNA from sample of western blot?

Platini Kwok platinik at glink.net.hk
Sun Aug 9 19:10:40 EST 1998


> Hi,
>
> Recently, I was involved in doing western blot on retina of SD rat and
> I
> found that there was a lot sticky, jelly like structure after boiling
> with TBS with SDS, triton x-100 buffer although I centrifuged it can
> sonicate the supernatant, it still here. How should I do so that I can
>
> get rid of it? These sticky material gave vertically line rather than
> horizontal band in SDS page or NC membrane.
>
> Thank you!







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