pET-Expression Mystery - The Sequel

Lars Komorowski larskomo at
Mon Aug 10 03:10:07 EST 1998

Indeed the protein which I am trying to express is very acidic - pI is
4-4.5 - and I am certain that there are no inclusion bodies during my
expression because the pellet doesn't look like those from other expressions
which form inclusion bodies (white an huge). So one could guess that the
protein is disintegrated during purification which seems to be very fast
since the purifaction takes only 2 hours. Who has experienced similar things

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