help! binding to nickle column

liz liz at METHYLGENE.COM
Thu Aug 13 15:24:33 EST 1998


I am trying to bind a 180 KDa protein with a His tag to a nickle column
(invitrogen) but most of the protein comes out in the flow through.  I
have tried both native and denaturing conditions.  Can anybody offer any
suggestions?



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