Transient protein interactions
Dr Engelbert Buxbaum
eb15 at le.ac.uk
Fri Aug 14 13:02:33 EST 1998
Alex Hutagalung wrote:
> Could anyone please suggest the best way to show an interaction between
> proteins that transiently interact? Any suggestions would be terrific.
> Thank you.
Crosslinking with bifunctional reagent (check Pierce catalogue for a
good selection). Emanescent wave detectors (like a Pharmacia BiaCore).
Analytical ultracentrifugation or gelchromatography. A genetic approach
would be the yeast two hybrid system. Low angle laser light scattering
or neutron scattering. Radiation target size analysis (not my prefered
option, though). You may try to grow crystals of the associated proteins
(if that actually works, it would be the ideal solution). CD spectrum of
the protein complex may differe from what is expected from a mixture of
the two proteins, indication conformational changes upon binding.
Well, at least that's what I can think of at the top of my head. Get a
good book on physical biochemistry, and you may find further options.
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