Thrombin Cleavage of GST Fusions

Michael DiDonato michael.didonato at
Mon Aug 17 13:09:41 EST 1998

Does anyone know of a way to optimize thrombin cleavage of GST fusion such
that only the five base site is cleaved and not the three base sites.  I
am getting the majority of cleavage at the five base site but also some
annoying cleavage at the three base sites present in my protein.  I have
heard of using heparin in the cleavage reaction, does anyone know of this?

Any help would be appreciated.


Michael DiDonato
Structural Biology and Biochemistry
The Hospital for Sick Children
Toronto, Ontario, Canada

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