microwave hydrolysis on PVDF membranes
Patricia W. Cash
CASH at bms.com
Tue Aug 25 10:14:14 EST 1998
We are trying to hydrolyze proteins on a PVDF membrane using a
microwave. Using our normal protein hydrolysis conditions
(vapor-phase HCl, 110 PSI), the membrane "melts" or becomes clear and
hard. We have found that if the pressure is not allowed to exceed 60
PSI, the membrane generally remain intact. However, we are not
getting complete hydrolysis.
Has anyone ever hydrolyzed proteins bound to PVDF membranes in a
microwave? Any tips to overcome the "melting" would be appreciated.
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