western-quantitation??

Martin Offterdinger a8803349.nospam at unet.univie.ac.at
Wed Aug 26 08:29:17 EST 1998


Hi!
I would like to know if you would consider it as science to quantitate
bands obtained by enzymatic Western Blotting with a densitometer. I
would like to study the expression of a certain protein under the
influence of hormones and compare it to control treated cell extracts.
I know if you put the film under the densitometer you will get numbers
(per cent of control) anyway. But is it good practice to conclude from
the relative band intensity in such an experiment to the relative
protein expression or is it just numbers that do not mean anything?
I am looking forward to discussion.
martin
Martin Offterdinger
Internal Med.I,Dept. Oncology
University of Vienna
Austria
E-Mail:a8803349.nospam at unet.univie.ac.at
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