microwave hydrolysis on PVDF membranes

Dr Engelbert Buxbaum eb15 at le.ac.uk
Fri Aug 28 14:47:56 EST 1998


Patricia W. Cash wrote:
> 
>      Hi,
> 
>      We are trying to hydrolyze proteins on a PVDF membrane using a
>      microwave.  Using our normal protein hydrolysis conditions
>      (vapor-phase HCl, 110 PSI), the membrane "melts" or becomes clear 

There was a paper some time ago in Anal. Biochem. on using alkaline
conditions for protein hydrolysis. As far as I remember the main
advantage claimed was that you do not need to use pressurized vials.



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