atunni at imb-jena.de
Mon Aug 31 08:38:10 EST 1998
We currently have a protein solution which is 99% (plus) pure,
however we have discovered protease activity within our sample.
This protease activity can be prevented by addition of EDTA
and using several protease inhibitors.
The question I have, is how can I discover if the protein we are working
has intrinsic protease activity (it is not supposed to) or if the less
1% impurity is infact a protease ?
We have tried silver stained gels
and Native gels to see if we can detect the contaminant.
Any advice or help would be greatly appreciated
atunn at imb-jena.de
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