FPLC - Superdex 200HR vs. Superose 12HR columns

Lloyd Graham lloyd.graham at REMOVE-MEmolsci.csiro.au
Thu Dec 3 01:03:52 EST 1998

Can anyone comment on relative advantages (for protein size estimation and
purification) of Pharmacia's new(ish) Superdex-200HR 10/30 column, as
compared to their long-standing Superose-12HR 10/30 column? They both have
similar nominal separation ranges (which are suitable to our needs) and
both appear to have comparable theoretical plates & and flowrates despite
having different matrix compositions. Feedback gratefully received.



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