FPLC - Superdex 200HR vs. Superose 12HR columns

Bassie baslee at kabelfoon.nl
Thu Dec 3 04:26:37 EST 1998


Lloyd Graham wrote:

> Can anyone comment on relative advantages (for protein size estimation and
> purification) of Pharmacia's new(ish) Superdex-200HR 10/30 column, as
> compared to their long-standing Superose-12HR 10/30 column? They both have
> similar nominal separation ranges (which are suitable to our needs) and
> both appear to have comparable theoretical plates & and flowrates despite
> having different matrix compositions. Feedback gratefully received.
>
> Thanks,
>
> Lloyd.
>

Lloyd,

In my experience the Superose 12 behaves better and with sharper peaks. I use
this one for analytical purposes. The Superdex 200 has a somewhat less
separation behaviour and thus I use this one for small scale preparative
purposes.

Regards

---=== Bassie ===---




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