FPLC - Superdex 200HR vs. Superose 12HR columns

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Dec 3 10:42:19 EST 1998


In article <lloyd.graham-0312981609030001 at tastiger.dbe.csiro.au>, lloyd.graham at REMOVE-MEmolsci.csiro.au (Lloyd Graham) wrote:
>Can anyone comment on relative advantages (for protein size estimation and
>purification) of Pharmacia's new(ish) Superdex-200HR 10/30 column, as
>compared to their long-standing Superose-12HR 10/30 column? They both have
>similar nominal separation ranges (which are suitable to our needs) and
>both appear to have comparable theoretical plates & and flowrates despite
>having different matrix compositions. Feedback gratefully received.
>

They are indeed very similar. In my experience limited to only several
runs on both, Superdex has a bit wider separation range and yields of
activities were higher. 

        - Dima



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