Cupper staining of gels

kll at crc.dk kll at crc.dk
Mon Dec 7 05:21:32 EST 1998


Could anybody tell me how to do a (*rapid*) copper staining  of a SDS-PA gel.
Will any quality of CuCl_2 do?
Can the CuCl_2 sol. be reused (how many times)?
The protocol I have simply says that I should rinse the gel in H_2O and then
soak it in 0.3 M CuCl_2 for appox. 5 min.
Any other tricks which will improve efficiency?

Thanks
Kresten

--
Kresten Lindorff Larsen
Dept. Yeast Genetics
Carlsberg Laboratory
Denmark

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