Cupper staining of gels

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Mon Dec 7 06:54:34 EST 1998


kll at crc.dk wrote:
> The protocol I have simply says that I should rinse the gel in H_2O and then
> soak it in 0.3 M CuCl_2 for appox. 5 min.

Worked for me (yes, it's really that simple!). I think reusing the CuCl_2
solution may be not a good idea because it becomes enriched in SDS. I am
not sure whether CuSO_4 will do the trick as well. I used some old CuCl_2
from Sigma that I found on the shelves.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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