Imidazol induced oligomerization?
klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Tue Dec 8 09:16:02 EST 1998
In article <366D1A38.9FF502B9 at pasteur.fr>, Pierre Rodrigues <pirod at pasteur.fr> wrote:
>Does anybody knows if imidazol (used as eluant of his tag purification)
>can induce or at least strongly stabilize protein oligomerization?
>This because when I purify a receptor by histag technique I always
>obtain it as if it was an oligomer (detected by western-blot), even
>resistant to SDS-PAGE. When I look at it directly by western-blot on
>crude extracts I have it mainly as a monomer with some higher bands. So,
>could imidazol have stabilize the interaction?
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