Iodo acetamide blocking

[Henning Pluecken]

Hello everyone,

does anyone know a recipe for blocking reduced SH-groups of proteins by
using iodoacetamide in protein samples prior to loading them on an SDS-PAGE?

Thanks for your help,

Henning Pluecken
Institut fuer Entwicklungs- und Molekularbiologie der Pflanzen
Heinrich-Heine-Universitaet Duesseldorf
Universitaetsstrasse 1

40225 Duesseldorf

pluecken at mail.rz.uni-duesseldorf.de