actin blotting

Pierre Rodrigues pirod at pasteur.fr
Fri Dec 11 04:11:42 EST 1998


Hi!

I am trying to detect actin in western-blot after several treatments. I
am using sigma AC-40 mAb. I can see in the blots a few additional bands
at higher MW, which appear to be multiples of actin monomer. Can anybody
tell me if these bands could be specific, or could be due to
cross-reaction of the Ab with some parasitic band (when using the AC-40
Ab)?
Is there any way to detect actin small order oligomers by gentle
SDS-PAGE/WB of cell extracts?

thanks
Pierre Rodrigues




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