Imidazol induced oligomerization?

Christian Radauer rc150295 at
Fri Dec 11 13:38:06 EST 1998

Dima Klenchin wrote:
> In article <366D1A38.9FF502B9 at>, Pierre Rodrigues <pirod at> wrote:
> >Hello,
> >
> >Does anybody knows if imidazol (used as eluant of his tag purification)
> >can induce or at least strongly stabilize protein oligomerization?
> >
> >This because when I purify a receptor by histag technique I always
> >obtain it as if it was an oligomer (detected by western-blot), even
> >resistant to SDS-PAGE. When I look at it directly by western-blot on
> >crude extracts I have it mainly as a monomer with some higher bands. So,
> >could imidazol have stabilize the interaction?
> NO.


Indeed it is not the imidazol that causes oligomerization, but the
His-tag, which is crosslinked by heavy metal ions. I have not tried it
yet, but maybee adding EDTA to the PAGE sample buffer would help.

Christian Radauer
Institute of General and Experimental Pathology
University of Vienna, Austria

More information about the Proteins mailing list