actin blotting

Annette C. Hollmann ah690549 at bcm.tmc.edu
Sun Dec 13 14:25:04 EST 1998


In article <3670E1CA.D348A71E at pasteur.fr> Pierre Rodrigues <pirod at pasteur.fr> writes:
>Hi!
>
>I am trying to detect actin in western-blot after several treatments. I
>am using sigma AC-40 mAb. I can see in the blots a few additional bands
>at higher MW, which appear to be multiples of actin monomer. Can anybody
>tell me if these bands could be specific, or could be due to
>cross-reaction of the Ab with some parasitic band (when using the AC-40
>Ab)?
>Is there any way to detect actin small order oligomers by gentle
>SDS-PAGE/WB of cell extracts?

I have tried to use this same antibody for Western blot, and never did get
it to work. AC-40 works marvellously for immunofluorescence, but appears
to be useless for Western blots.

Annette


















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