DNA binding protein purification

Joel Baguet Joel.Baguet at ens-lyon.fr
Mon Dec 14 11:37:15 EST 1998


I need to purify some proteins which bind to DNA. I use DNA binding
protein purification kit from Boerhinger with long concatamers of 
protein-binding
oligonucleotide (obtainrd using self-primed PCR technique) ligated
streptavidin magnetic particles. The protein-binding conditions are
those for gel retardation assay.

The results are very poor (about 1% with 35S in vitro synthetized
proteins).

Does anybody have some experience about that??



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