Fusion protein cleavage

kll at crc.dk kll at crc.dk
Wed Dec 16 06:41:59 EST 1998


I am going to make a fusion protein for purification purposes and I would like
to know about good and bad experiences with different types of cleavage
enzymes. As far as I know the most popular proteases for cleavage are:

Thrombin
Factor Xa
Enterokinase

Can anybody recommend any of these (or other) enzymes with regards to
effectivity, specificity, price, removal of protease etc.

Thanks
Kresten

--
Kresten Lindorff Larsen
Dept. Yeast Genetics
Carlsberg Laboratory
Denmark

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