prevelig at uab.edu
Fri Jan 9 06:08:23 EST 1998
In article <34B5988F.41C6 at interzon.com>, Uncle S <UncleS at interzon.com> wrote:
>Lion wrote in this pop quiz:
>> How many methods are available for assaying protein-protein interaction?
>Just two, I'm afraid.
>> What are these methods?
>Well, there's the yeast two hybrid (Tm) and the other one doesn't
there are a whole host of quantitative methods as well. I don't off hand
know of a good reference but there may be one out there. I will just list
a few for you here.
analytical ultracentrifugation - perhaps the best, both velocity and
BIACore and other plama resonsance techniques - quantitative? well you be
the judge :)
hydrodynamic techniques - column chromatography, large zone and traditional
spectroscopic techniques - CD,fluorescene etc....changes in signal with
equilibrium dialysis - if one protein/ligand is small enough
this is a quick list, i can probably think of half a dozen other
techniques. If you are interested in a particular system, feel free to
drop me some mail.
Associate Professor, Dept. of Microbiology
Univ. of Alabama @ Birmingham
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