T7 RNA polymerase phage?

Wolfgang Resch wresch at email.unc.edu
Fri Jan 9 10:03:06 EST 1998


novagen sells recombinant lambda phage (CE6) used with their pET
expression system. an aliquot of high titer phage enough to start growin
your own stock and some more costs about 50 $. buying enough for
infections is too expensive.
also did you try BL21 DE3 pLysS or pLysP ? more stringent control of the
polymerase by expressing small amount of lysozyme from a plasmid. also has
the advantage that these cells lyse when you freeze-thaw them because of
the lysozyme.

wolfgang




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