protein-protein interaction

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Jan 9 09:54:30 EST 1998


Lion <hccha at ms9.hinet.net> wrote:
> How many methods are available for assaying protein-protein interaction?
> What are these methods?

Off the top of my head:

* Two-hybrid system and its recent modifications
* Coimmunoprecipitation
* Far western blotting
* BIAcore technology
* ELISA
* Analytical gel filtration

Many of these technologies are variations on a theme: one protein is
immobilized, and the interaction of the second protein with the solid
phase is detected. Analytical gel filtration detects protein-protein
interaction in liquid phase, but the affinity must be quite high to
find the complex. Two-hybrid system is a genetic in vivo approach.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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