T7 RNA polymerase phage?

Christian Ebeling cebelin at gwdg.de
Sat Jan 10 23:41:55 EST 1998


Wolfgang Resch wrote:
>
>also did you try BL21 DE3 pLysS or pLysP ? more stringent control of the
>polymerase by expressing small amount of lysozyme from a plasmid. also has
>the advantage that these cells lyse when you freeze-thaw them because of
>the lysozyme.
 This ( pLysS) works great for expresssion of toxic proteins and you will
have less transformation problems.
Christian

------------
Christian Ebeling
Institut fuer Moleculare Genetik
Universitaet Goettingen
Germany
http://www.gwdg.de/~cebelin






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