Expressing the cpp32 in yeast nucleus

Dooyeon Kim dykim at bioneer.kaist.ac.kr
Sun Jan 11 22:20:31 EST 1998


Hi, Netters.

I am trying to express the cpp32(caspase-3) in yeast nucleus.

The protease(caspase-3) expressed was designed to contain SV40

nuclear localize signal in its N terminal.

The problem is 

1. The protease is activated in the yeast by proteolysis of the middle

of the protein. Although the cleaved fragment is not dissociated(actually

interacts with other fragments to become a complex), I wonder if the SV40

nuclear localize signal(fused in the N-terminal) is working.

2. Caspase-3 is known to exist in cytosol and nucleus. Is it possible to

make this protease exist only in nucleus?

Any information or advice regarding above problems will be greatly appreciated.

Thanks in advance. 
Bye.

--

___________________________________________________________________________
   
KAIST, Daejon Korea.                     Dooyeon Kim ......................
                                         dykim at bioneer.kaist.ac.kr
___________________________________________________________________________


--

___________________________________________________________________________
   
KAIST, Daejon Korea.                     Dooyeon Kim ......................
                                         dykim at bioneer.kaist.ac.kr
___________________________________________________________________________




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