Protein expression with the IMPACT system

frankenberg frnicole at
Mon Jan 19 14:16:30 EST 1998

has anybody experience with protein expression using the IMPACT system
from NEB? I have a very low expression level, tried already different
media, temperature of induction, amount of IPTG. Does anybody has a
useful hint? Did someone express a protein successfully and in large
Thanks already for replies.

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