Glysosylation and western blot

Bruce G. Allen allen at cardio.ICM.UMontreal.CA
Wed Jan 21 18:00:45 EST 1998


Sophie,
What did you use to determine the MWt of your protein? Is it 
calculated? Determined by gel filtration? Glycosylation will affect the 
mobility on SDS-PAGE. However, so will extremes of pI, shape, and a few 
other things.
Bruce

Bruce G. Allen, Ph.D.                          Phone: 514-376-3330 (3591) 
Institut de Cardiologie de Montreal            FAX:   514-376-1355
Centre de Recherche                            EMAIL: allen at icm.umontreal.ca 
5000, rue Belanger est
Montreal, Quebec
Canada
H1T 1C8




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