Glysosylation and western blot
stu30142 at mail.uni-kiel.d400.de
Thu Jan 22 13:28:24 EST 1998
"Christophe GUENIN" <christophe.guenin at hol.fr> wrote:
>I would like to know when a protein have a site of glycosylation.
>Does anybody know if its migration in gel SDS is more quickly ?
>My protein have a molecular weight of 51 kDa.
>With a gel she has a molecular weight of 40 kDa.
>Does anybody know the phenomenon ?
>Thanks to give me explanation and bibliographic references.
>christophe.guenin at hol.fr
I am working with glycosylated Albumin and the glycosylation increases
the MW in SDS-PAGE. It is commonly thought that this behaviour is due
to interaction of the glycosylated protein with the gelmatrix rather
than caused by a real increase in MW. If your protein seems to have a
lower MW after glycosylation, it maybe has changed its confirmation.
Kai Lehmann stu30142 at mail.uni-kiel.d400.de
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