Protein folding and Mr estimates

Thomas J. Dougherty, Ph.D. Thomas_J._Dougherty at ccMail.bms.com
Fri Jan 23 21:07:44 EST 1998


Cormac Shaw wrote:
> 
> Hi folks,
> 
> Semi-hypothetically speaking, what's the likelihood of this?:
> 
> Two similar enzymes with essentially the same polypeptide chain length
> and amino acid composition but with different folding conformations.
> Niether are glycosylated.
> BUT the two have apparent relative molecular masses
> of 74,000 and 79,000 on SDS-PAGE after treatment with
> mercaptoethanol.
> 
> Is this probable/possible/crazy? Any pointers towards references of
> such occurances and/or comments very welcome. Maybe I'm just tired.
> 
> Cormac.
________________________________________________________________________
> Cormac Shaw, BSc,                 |
> Dept. of Industrial Microbiology, |  mailto:Cormac.Shaw at ucd.ie
> University College Dublin,        |   phone: +353 (1) 706 1307
> Dublin 4, Ireland                 |     fax: +353 (1) 706 1183
> -__

There are several cases of anomalous migration of proteins in denaturimg
SDS gels that I've seen or read about.  Actually, your two proteins
don't appear to be too disparate in apparent MW.  
Tom



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