Anti-histag antibodies etc

Thomas urbig at biokemi.su.se
Mon Jul 6 07:10:47 EST 1998


In article <359A5802.6267749C at nott.ac.uk>, Simon.Dawson at nott.ac.uk wrote:

> Hi all,
>                 I was wondering if anyone out there knows what
anti-histag type antibodies
> are available and which give the best results. I have the M2 anti-flag
> antibody from Kodak/IBI and the anti-Xpress antibody from Invitrogen.
>         I seem to remember a while back that someone out there knew of
the existence
> of an HRP-NTA conjugated antibody that bound directly to the histag and can be
> detected with an HRP substrate. Was I imagining that?? If anyone can confirm
> this and knows where I can get hold of such an antibody, could they let me
> know? It would be much appreciated. Thanks.
> 
> 
>                         Simon.


Qiagen produces a Ni+-NTA coupled directly to HRP or AP. I tried the
AP-conjugate and it worked fine. The 4HIS and 5HIS ab´s are also good
(immunprecip!) but for standard westerns the conjugate gives faster
results and great signal to noise ratio.

Thomas

-- 
Thomas Urbig
urbig at biokemi.su.se



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