How much Protein needed for sequencing?

[Christoph Peter]

Hi dear scientific community!

What is the minimum amount of protein, in micrograms (50kDa) or
picomoles, on a SDS-PAGE (or western-blotted) which could still be
reliably sequenced by a state-of-the-art-method? 

Many thanks in advance............................Christoph
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Christoph Peter
PhD Student (Biotechnology)
cpeter at zedat dot fu-berlin dot de

>Alles wird gut!<(Beate)