endoproteinase glu c

[Jonathan B. Marder]

>Has anyone used Boehringer Mannheim's endoproteinase glu c sequencing
grade?
> If so did anyone have success resuspending this enzyme in water or
some
>other volatile buffer like ammonium carbonate and freezing for use at
later
>date?


This is V8 protease right? If so, I have good experience keeping it as
frozen stock (10 mg/ml in water)
at -80 celcius.

Jonathan B. Marder   <MARDER at agri.huji.ac.il>
Department of Agricultural Botany, The Hebrew University of Jerusalem
Faculty of Agriculture, P.O.Box 12, Rehovot 76100, ISRAEL
Phone: +972 8 9481918   Fax:   +972 8 9467763
Web page:    http://www.agri.huji.ac.il/~marder