excising/concentrating protein bands
atutter at aim.salk.edu
Mon Jul 27 22:47:10 EST 1998
i'm new to this group, i hope my question is not inappropriate:
does anyone have a tried-and-true protocol for excising a protein band from
a gel, electroeluting it onto a membrane, and precipitating it so as to
concentrate it? i would like to silver stain the gel first if
re-solubilization is possible. the key here is minimal loss. i am trying
to concentrate a peptide that i am pulling down from nuclear extract using a
his-tagged protein pulldown protocol. the band is detectable by silver
stain. i need to load at least 50 or so lanes to have enough peptide to
obtain sequence data.
please e-mail me your reply, i don't have access to the newsgroup form work.
thanks in advance,
Salk Institute for Biological Studies
10010 N. Torrey Pines Rd.
La Jolla, CA 92037
email: atutter at aim.salk.edu
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