excising/concentrating protein bands

[Antonin Tutter]

Hi all.

i'm new to this group, i hope my question is not inappropriate:

does anyone have a tried-and-true protocol for excising a protein band from
a gel, electroeluting it onto a membrane, and precipitating it so as to
concentrate it?  i would like to silver stain the gel first if
re-solubilization is possible.  the key here is minimal loss.  i am trying
to concentrate a peptide that i am pulling down from nuclear extract using a
his-tagged protein pulldown protocol.  the band is detectable by silver
stain.  i need to load at least 50 or so lanes to have enough peptide to
obtain sequence data.  

please e-mail me your reply, i don't have access to the newsgroup form work.

thanks in advance,



_______________________________________
Antonin Tutter
Salk Institute for Biological Studies
RBIO-J
10010 N. Torrey Pines Rd.
La Jolla, CA  92037
email:  atutter at aim.salk.edu
web:  http://www-biology.ucsd.edu/~atutter/