murphy_r at licre.ludwig.edu.au
Tue Jul 28 19:30:39 EST 1998
In the past I've used dialysis tubing to concentrate proteins by merely
hanging them in an air stream - like in a fume hood and leave the motor
running. I guess this is a variation on what you're thinking of. It worked
quite nicely (volume reduction of ~80%), but be careful of precipitation
problems of either your protein or salts if you caary it too far.
Hope this helps,
In article <i.mcfarlane-2807981850060001 at mac017044.guys.icnet.uk>,
i.mcfarlane at icrf.icnet.uk (Ian McFarlane) wrote:
>Once upon a time somebody mentioned that there was some trick with
>dialysis tubing and vaccuum that could be performed to concentrate
>proteins (flash dialysis?). Does anyone know how to do this or is my
>memory failing me?
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Heidelberg, Vic. 3084
Email murphy_r at licre.ludwig.edu.au
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