Vaccuum dialysis?

[Roger Murphy]

Ian,

In the past I've used dialysis tubing to concentrate proteins by merely 
hanging them in an air stream - like in a fume hood and leave the motor 
running.  I guess this is a variation on what you're thinking of.  It worked 
quite nicely (volume reduction of ~80%), but be careful of precipitation 
problems of either your protein or salts if you caary it too far.

Hope this helps,

Roger


In article <i.mcfarlane-2807981850060001 at mac017044.guys.icnet.uk>, 
i.mcfarlane at icrf.icnet.uk (Ian McFarlane) wrote:
>Once upon a time somebody mentioned that there was some trick with
>dialysis tubing and vaccuum that could be performed to concentrate
>proteins (flash dialysis?). Does anyone know how to do this or is my
>memory failing me?
>
>Thanks,
>
>Ian Mc



Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg,  Vic. 3084
Australia.

Tel  61-3-94965463
Fax  61-3-94965436
Email murphy_r at licre.ludwig.edu.au