Vaccuum dialysis?

noone noone at cancer.bham.ac.uk
Wed Jul 29 08:36:43 EST 1998


In article <6pltqb$kua$1 at izvestia.its.unimelb.edu.au>,
murphy_r at licre.ludwig.edu.au (Roger Murphy) wrote:

> Ian,
> 
> In the past I've used dialysis tubing to concentrate proteins by merely 
> hanging them in an air stream - like in a fume hood and leave the motor 
> running.  I guess this is a variation on what you're thinking of.  It worked 
> quite nicely (volume reduction of ~80%), but be careful of precipitation 
> problems of either your protein or salts if you caary it too far.
> 
> Hope this helps,
> 
> Roger



or alternatively, you can use aquacide (a highly hygroscopic powder) and
place your bag in it. Works quite nicely,

Peter



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