SDS and Ni2+ resin

Ian McFarlane i.mcfarlane at icrf.icnet.uk
Wed Jul 29 11:01:17 EST 1998


What effect does SDS have on Ni2+ metal affinity columns. Does it merely
interfere with the binding of the protein to the column or does it have
some irreversible effect which would lead to the column being useless?

Ian Mc



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