SDS and Ni2+ resin

Vladimir Rotrekl rotrekl at chemi.muni.cz
Thu Jul 30 05:47:27 EST 1998


Ian McFarlane wrote:

> What effect does SDS have on Ni2+ metal affinity columns. Does it
> merely
> interfere with the binding of the protein to the column or does it
> have
> some irreversible effect which would lead to the column being useless?
>
> Ian Mc

   It depends on resign, but generally no damage should be detectable in
low SDS concentration.
For example Ni-NTA agarose and superose (from Qiagen) require for
regeneration 2% SDS (recommended by manufacturer).

Vladimir
-------------- next part --------------
A non-text attachment was scrubbed...
Name: vcard.vcf
Type: text/x-vcard
Size: 453 bytes
Desc: Card for Vladimir Rotrekl
Url : http://iubio.bio.indiana.edu/bionet/mm/proteins/attachments/19980730/64a968b9/vcard.bin


More information about the Proteins mailing list