Ligand Binding

Dr E. Buxbaum EB15 at le.ac.uk
Mon Mar 2 13:22:52 EST 1998


Louis Hom wrote:
> 
> Basic question:
>         Is there an activation energy associated with ligand binding?

Yes, there is, and it is easy to measure. Simply determine the Kd at
various temperatures and plot ln(Kd) vs the reciprocal temperature
(absolute temperature, i.e. in Kelvin). If certain conditions are met,
then the data points will be on a straight line, from the slope of which
you can calculate the activation energy.



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