Dr E. Buxbaum
EB15 at le.ac.uk
Mon Mar 2 13:15:46 EST 1998
Deirdre Sholto-Douglas wrote:
> He describes using a Calcium phosphate gel column and the addition
> of stepwise phosphate buffers to elute the coloured proteins from
> _Porphyra_. His methodology states that Celite was mixed to the
> gel "as support" but its unclear exactly how much was used.
> Admittedly, the gel is decidedly unstable as is and would probably
> benefit from the addition of diatomaceous earth.
> Has anyone done something similar or know the rough proportions
> of such a mixture?
If by Calcium phosphate you mean Hydroxyapatite, then there are nice
beaded forms of this material on the market, for example from BioRad. An
introduction into HA chromatography can be found in Deutscher's "Guide
to Protein Purification" (in the Methods in Enzymology series).
More information about the Proteins