Staining of PVDF after transfer?

Junghans Junghans at
Tue Mar 3 09:08:03 EST 1998

I have a problem with staining of PVDF-membranes after western-blot
How can I stain the marker or in general my proteins before
immunodetection. I tried PonceauS but this didn't work even after adding
some methanol the result was not very promising, because only very
strong bands were recognized.
Does anybody know a more sensitive method?
Thanks, Dirk Junghans
Dirk Junghans
Max-Planck-Institut f. Hirnforschung
Abt. Neurochemie
Deutschordenstr. 46
60528 Frankfurt am Main

e-mail: junghans at
Tel. ++49 - 69-96769-457
Fax. ++49 - 69-96769-441

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